Last edited by Malagor
Thursday, May 14, 2020 | History

4 edition of Microinjection found in the catalog.

Microinjection

methods and applications

by David J. Carroll

  • 25 Want to read
  • 29 Currently reading

Published by Humana Press, Springer [distributor] in New York. N.Y, [London] .
Written in

    Subjects:
  • Microinjections

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by David J. Carroll
    SeriesSpringer protocols, Methods in molecular biology -- 518
    Classifications
    LC ClassificationsQH585.5.M52
    The Physical Object
    Paginationxvi, 221 p. :
    Number of Pages221
    ID Numbers
    Open LibraryOL25571930M
    ISBN 101588298841, 1597452025
    ISBN 109781588298843, 9781597452021
    LC Control Number2008938642
    OCLC/WorldCa166372375

    With an inherent immediacy, microinjection has facilitated a wide range of biological studies, some of which are described in this book. While numerous alternative “microinjection” tools have also been developed, such as vesicle fusion, scrape loading and electroporation, we will focus mainly on the versatility of the use of needles to Cited by: 6. Microinjection is the process of transferring genetic materials into a living cell using glass micropipettes or metal microinjection needles. Glass micropipettes can be of various sizes with tip diameters ranging from to 10 µm. DNA or RNA is injected directly into the cell’s nucleus. Microinjection has been successfully used with large.

    THE ZEBRAFISH BOOK A guide for the laboratory use of zebrafish Danio (Brachydanio) rerio by Monte Westerfield, Institute of Neuroscience, University of Oregon.   T1 - Microinjection of Xenopus embryos. AU - Garriock, R. J. AU - Krieg, P. A. PY - /1/1. Y1 - /1/1. N2 - INTRODUCTION Microinjection of Xenopus embryos is an important technique with multiple applications in the fields of Cell Biology and Developmental : R. J. Garriock, Paul A Krieg.

      Since there is a new microinjection laboratory working onthis illing and microinjection were developed jointly, improving theirsynergies and . The microinjection technique is a direct physical approach, and therefore host‐range independent, for introducing substances under microscopical control into defined cells without damaging them. These two facts differentiate this technique from other physical approaches, such as biolistic transformation and macroinjection (see chapters in Cited by:


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Microinjection by David J. Carroll Download PDF EPUB FB2

This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in : Hardcover. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal Format: Hardcover.

Book Condition: This is an ex-library book and may have the usual library/used-book markings book has soft covers. With usual stamps and markings, In good all round condition. Please note the Image in this listing is a stock photo and may not match the covers of the actual itemAuthor: Juan Carlos Lacal.

In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal.

Description: Written by expert researchers, this book presents methods using microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the goal of learning about gene expression, signal transduction, and Microinjection book function within living cells.

There are adequate black and white illustrations and : $ This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically Microinjection book species that can broadly impact our society in general.

Buy MICROINJECTION. on FREE SHIPPING on qualified orders MICROINJECTION.: Rosario Perona, James Feramisco Juan Carlos Lacal: : Books. The First European Workshop on Biotechnology Applications of Microinjection, Microscopic Imaging and Fluorescence was run at the University of East London, U.K, 21stth April, with the objective of bringing together a diverse group of individuals who were using these state-of-the-art applications for biotechnological exploration.

A novel Format: Paperback. About this book Introduction In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells.

Microinjection Microinjection is a technique of delivering foreign DNA into a living cell (a cell, egg, oocyte, embryos of animals) through a glass micropipette. One end of a glass micropipette is heated until the glass becomes somewhat liquified.

It is quickly stretched which forms a. Introduction to Microinjection. and its position is interpreted by software which in turn drives three motors set at right angles to each other, rep-resenting the three major geometric axes, X, Y and Z (see figure below).

The advantages of electronic motor micro-File Size: KB. Microinjection. Editors: Lacal, Juan Carlos, Perona, Rosario, Feramisco, James (Eds.) Free PreviewBrand: Birkhäuser Basel. Microinjection is an essential approach in the study of mammalian oocytes and early embryos, and is useful for the introduction of many molecules and reagents.

Whereas microinjection into germinal vesicle stage oocytes is relatively simple using various microinjection setups, metaphase-II mouse eggs are notoriously fragile, and nondamaging microinjection can be difficult to achieve. DNA transformation and microinjection are essential tools for C.

elegans research. Transformation is used to clone genes by mutant rescue, to over-express or ectopically express genes, to express tagged proteins, to study structure/function of protein domains, and to analyze DNA or RNA regulatory elements.

Furthermore, transgenes. Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations. In DNA microinjection, also known as pronuclear microinjection, a very fine glass pipette is used to manually inject DNA from one organism into the eggs of another.

The best time for injection is early after fertilization when the ova have two pronuclei. In this book expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of.

elegans microinjection. Contributed by Ian Chin-Sang, Queens University, ON, Canada. One way to make transgenic animals in C. elegans we use a microinjection technique. Briefly, a DNA construct (plasmid, cosmid or YAC) or PCR product with your gene(s) of interest is mixed with a co-injection marker and injected into the distal gonad (syncytium).

"The technique of needle microinjection has become a prominent experimental approach in biological research."--BOOK JACKET.

"This book presents information on the technique's principles, the required equipment, preparation of recipient cells and injected material and the analysis of the results. Microinjection is the use of a glass micropipette to inject a liquid substance at a microscopic or borderline macroscopic level.

The target is often a living cell but may also include intercellular space. Microinjection is a simple mechanical process usually involving an inverted microscope with a magnification power of around x (though sometimes it is performed using a dissecting stereo.

The Use of Plasmid Microinjection to Study Specific Cell Cycle Phase Transitions. E. S. Knudsen. Pages About this book. Keywords.

activation apoptosis cell cell cycle cytoskeleton DNA gene membrane membrane transport Peroxisom protein proteins receptor regulation RNA.Microinjection is an essential technique with a wide range of applications. Therefore, the book addresses a large audience.

It features a shared European / American editorship and describes methodology and applications of microinjection, a powerful technique that is an indispensible approach today for a wide range of questions in cell and molecular biology.ISBN: OCLC Number: Description: xiii, pages: illustrations (some color) ; 25 cm: Contents: Part 1 Strategies and Protocols for Microinjection Studies Using Somatic Cells in Culture Protocols for the Manual and Automatic Microinjection of Somatic Cells in Culture and for the Analysis of Microinjected Cells, Angel Cid-Arregui, Carla Santana, Miriam.